{"created":"2023-05-15T13:57:26.625703+00:00","id":140,"links":{},"metadata":{"_buckets":{"deposit":"18b5f478-e6e5-42ba-91d1-8c9d332ecfde"},"_deposit":{"created_by":16,"id":"140","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"140"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000140","sets":["1686642104765:1:12:15"]},"author_link":["500"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-09-18"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2015","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We cultured HMS0014 Yub621b cells within a 3D collagen gel scaffold (Cellmatrix Type I-A) and aimed to study the fate and contribution of human bone-derived mesenchymal stem cells (MSCs) in the guided bone regeneration(GBR)-engineered tissue which has developed around the titanium (Ti) test dental implant (IP) in vitro. The light microscopy (LM) and transmission electron microscopy (TEM) results of the peri-IP tissue indicated that collagen fibrils of the Cellmatrix Type I-A gel were accumulated and fabricated to provide a 3D meshwork for proliferation and differentiation of the HMS0014 cells in the top (cell) layer; mineralisation of the GBR tissue had commenced since day 1 and became markedly deposited between days 7 and 14 of the experiment. TEM observation revealed sedimentation of cement line at the periphery of the interwoven Cellmatrix fibres and fibrils in the ECM scaffold of the GBR tissue; matrix vesicle-mediated and appositional collagen-mediated mineralisation were identified in the peri-IP ECM scaffold. The fine structure study of the plurimorphic osteoblast(Ob)-like osteogeneic cells demonstrated numerous membranous organelles related with vesicular trafficking, secretion and endocytosis in the cytoplasm; well-developed cytoskeleton networks and intercellular junctional complexes were also observed. The specimens on fluorescence immunohistochemistry (IHC) by confocal laser-scanning microscopy (LSM) showed the expression of LC3 and Cx43 associated with autophagic-lysosomal degeneration pathway and signal conduction mediated with gap junctions (GJS) in maintaining tissue homeostasis of the Ob-like cells which grew and degenerated in the 3D scaffold. Results from this in vitro study suggest that Ob-like HMS0014 cells actively regulate turnover of the peri-IP ECM to recapitulate the development and formation of osteoid tissue-engineered material which might contribute to augment osseointegration around the dental implant.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙第1600号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森下, 愛子"},{"creatorName":"モリシタ, アイコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"500","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"otu_1600.txt.pdf","filesize":[{"value":"5.0 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/140/files/otu_1600.txt.pdf"},"version_id":"1dfded56-0b2e-44b0-be50-bf6f8079d522"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"otu_1600.pdf","filesize":[{"value":"212.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/140/files/otu_1600.pdf"},"version_id":"e07c101a-d7bf-4767-9d4c-f517fbdb03f3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Turn over","subitem_subject_scheme":"Other"},{"subitem_subject":"mesenchymal stem cells (MSCs)","subitem_subject_scheme":"Other"},{"subitem_subject":"3D collagen scaffold","subitem_subject_scheme":"Other"},{"subitem_subject":"GBR tissue","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type Ⅰ-A collagen gel scaffold in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type Ⅰ-A collagen gel scaffold in vitro"}]},"item_type_id":"10006","owner":"16","path":["15"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-01"},"publish_date":"2016-08-01","publish_status":"0","recid":"140","relation_version_is_last":true,"title":["A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type Ⅰ-A collagen gel scaffold in vitro"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-05-15T14:12:45.758554+00:00"}