{"created":"2023-05-15T13:57:21.041267+00:00","id":15,"links":{},"metadata":{"_buckets":{"deposit":"6a8f70f4-1083-4f5f-8c86-952d628a9f82"},"_deposit":{"created_by":14,"id":"15","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"15"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000015","sets":["1686642104765:1:6:8"]},"author_link":["21","425"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-06"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014年度","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第744号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"⽚⼭, 暢仁"},{"creatorName":"カタヤマ, ノブヒト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katayama, Nobuhito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"kou_744_txt..pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/15/files/kou_744_txt..pdf"},"version_id":"d6cab3d7-351d-4c49-91da-5ed1b2e42811"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"kou_744.pdf","filesize":[{"value":"186.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/15/files/kou_744.pdf"},"version_id":"12d2973c-d10c-4c6e-9565-74f84c32489e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Alkaline Phosphatase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell Proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cells, Cultured","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen Type I","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dental Enamel","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Flavonoids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mesenchymal Stromal Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oligopeptides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteocalcin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells"}]},"item_type_id":"10006","owner":"14","path":["8"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-01"},"publish_date":"2016-08-01","publish_status":"0","recid":"15","relation_version_is_last":true,"title":["The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells"],"weko_creator_id":"14","weko_shared_id":14},"updated":"2023-05-15T14:12:11.087947+00:00"}