{"created":"2023-05-15T13:57:27.178692+00:00","id":155,"links":{},"metadata":{"_buckets":{"deposit":"efe786ec-999d-42e3-97cf-33bdcb9fd21c"},"_deposit":{"created_by":16,"id":"155","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"155"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000155","sets":["1686642104765:1:16:17"]},"author_link":["525","526"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"単層あるいはスフェロイド培養下においてBMP-4によって刺激された脱分化脂肪細胞の初期軟骨マーカー遺伝子の発現挙動"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-10"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2016年度","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Human dedifferentiated fat (hDFAT) cells are thought to be a promising cell source for cartilage regeneration therapy. Nevertheless, the responses of hDFAT cells to bone morphogenetic proteins (BMPs) are still unclear. Here, we elucidated the effects of BMP-4 on the mRNA expression of early chondrogenic markers in hDFAT cells under monolayer or pellet cell culture conditions. \nMaterials and methods: Monolayer and pellet cell cultures of hDFAT cells were grown with control medium or chondrogenic medium (CM) with or without BMP-2, BMP-4, or BMP-7. Real-time polymerase chain reaction was used to analyze the mRNA expression levels of nine genes: chondrogenic markers, i.e., SOX9, SOX5, SOX6, aggrecan, type 2 collagen, type 10 collagen, and matrix metalloproteinase (MMP) 13; type 1 collagen; and MMP3. The BMP signaling inhibitor dorsomorphin was used to verify the mechanisms of BMP-4-induced chondrogenesis. \nResults: Recombinant BMP-4 (100ng/mL) increased the expression of SOX9, SOX6, and aggrecan mRNAs in monolayer cells compared with that in cells treated with BMP-2 or BMP-7 on day 3. Chondrogenically differentiated hDFAT cells induced by CM containing BMP-4 showed higher expression of eight genes (excluding SOX5) in monolayer cultures and nine genes (including SOX5) in pellet cultures compared with those in control medium on day 14. Dorsomorphin attenuated the effects of BMP-4. \nConclusion: These results showed that BMP-4 had the potential to modulate the early chondrogenesis of hDFAT cells under both monolayer and pellet cell culture conditions.\n","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第797号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安積, 瑛子"},{"creatorName":"アヅミ, エイコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"525","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Azumi, Eiko ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"526","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-04-20"}],"displaytype":"detail","filename":"kou_797_txt.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/155/files/kou_797_txt.pdf"},"version_id":"1b875a7c-1d58-4b28-a9e7-91b4c203602a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-04-19"}],"displaytype":"detail","filename":"kou_797.pdf","filesize":[{"value":"214.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/155/files/kou_797.pdf"},"version_id":"b5ac2a01-aab9-47ab-abd5-c6ac2c31c476"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Gene expression profiles of early chondrogenic markers in dedifferentiated fat cells stimulated by bone morphogenetic protein 4 under monolayer and spheroid culture conditions in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene expression profiles of early chondrogenic markers in dedifferentiated fat cells stimulated by bone morphogenetic protein 4 under monolayer and spheroid culture conditions in vitro"}]},"item_type_id":"10006","owner":"16","path":["17"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-19"},"publish_date":"2017-04-19","publish_status":"0","recid":"155","relation_version_is_last":true,"title":["Gene expression profiles of early chondrogenic markers in dedifferentiated fat cells stimulated by bone morphogenetic protein 4 under monolayer and spheroid culture conditions in vitro"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-05-15T14:18:17.390621+00:00"}