{"created":"2023-05-15T13:57:28.479006+00:00","id":178,"links":{},"metadata":{"_buckets":{"deposit":"98d6456b-4b3a-424c-a26a-b8387f6611a6"},"_deposit":{"created_by":16,"id":"178","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"178"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000178","sets":["1686642104765:1:33:28"]},"author_link":["560"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"高出力、赤色 light-emitting diode 照射は、ERK signaling 経路を介してヒト歯根膜幹細胞の増殖、骨分化および石灰化を増大する"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-09"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2017年度","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Light-emitting diode (LED) is attracting attention as a new light source for phototherapy. However, its effects on periodontal tissue regeneration remain unknown. The aim of this study was to examine the effects of high-power, red LED irradiation on human periodontal ligament stem cells (PDLSCs), which play an important role in periodontal tissue regeneration.\nMethods: PDLSCs were derived from adult human third molars. The light source was red LED (peak wavelength: 650 nm). Energy densities ranging from 0 to 10 J/cm2 were tested to determine the optimal dose. PDLSC proliferation was measured using two parameters: live cell protease and ATP levels. After the cells were induced to differentiate, Next, the effect of LED irradiation on osteogenic differentiation and mineralization was examined, with a particular focus on the extracellular signal-regulated kinase (ERK)1/2 signaling pathway using an ERK inhibitor (PD98059).\nResults: LED irradiation at 8 J/cm2 led to a significant increase in PDLSC proliferation and enhanced Runx2 and Osterix mRNA expression, ALP activity, procollagen type 1 C-peptide and osteocalcin production, calcium deposition and Alizarin Red S staining. In addition, LED induced the activation of ERK1/2, and the effects of LED on PDLSC proliferation, differentiation, and mineralization could be suppressed by treatment with PD98059.\nConclusion: The results of this study show that 650-nm high-power, red, LED irradiation increases PDLSCs proliferation, and osteogenic differentiation and mineralization, mediated by ERK1/2 activation. These findings suggest that LED may be a useful tool for periodontal tissue regeneration.\n","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第816号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山内, 伸浩"},{"creatorName":"ヤマウチ, ノブヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"560","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-12-18"}],"displaytype":"detail","filename":"kou_816_txt(accept version)1年後公開可.pdf","filesize":[{"value":"972.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/178/files/kou_816_txt(accept version)1年後公開可.pdf"},"version_id":"27dfeb81-cc02-408d-a29e-5f315d17a7a0"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-17"}],"displaytype":"detail","filename":"kou_816.pdf","filesize":[{"value":"199.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/178/files/kou_816.pdf"},"version_id":"87811f65-d9db-4aa5-bb5f-0e788c030235"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"periodontal ligament｜stem cells｜phototherapy｜low-level light therapy｜regeneration｜MAP kinase signaling system","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway"}]},"item_type_id":"10006","owner":"16","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-17"},"publish_date":"2018-05-17","publish_status":"0","recid":"178","relation_version_is_last":true,"title":["High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-05-15T14:25:08.774499+00:00"}