{"created":"2024-06-03T10:17:32.482419+00:00","id":2000089,"links":{},"metadata":{"_buckets":{"deposit":"2a01fea6-4f53-46ae-b36d-a4eafdb2ac92"},"_deposit":{"created_by":18,"id":"2000089","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000089"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000089","sets":["1686642104765:1:63:65"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-03-01"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Oral cancer is a common malignant cancer, which is difficult to treat with traditional methods. Photodynamic therapy is a new alternative that is gaining widespread attention. We investigated the therapeutic effectiveness against oral cancer cells, and the mechanism of action of near-infrared photodynamic therapy (NIR-PDT) combined with the photosensitizer Chlorin e6 (Ce6) and up-conversion nanoparticles NaYF4:Yb/Er (UCNPs). After using the MTT (3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide)assay to determine a suitable concentration combination, human squamous cell carcinoma cells (HSC-3s) were treated with NIR-PDT combined with Ce6 and UCNPs. We examined cell proliferation and death by MTT assay, cell membrane permeability by live or dead cell staining, apoptosis by the FITC-Annexin V/PI assay, intracellular singlet oxygen and reactive oxygen species (ROS). NIR-PDT with 0.5ng/µl Ce6 and 0.1ng/µl UCNPs inhibited the cell proliferation of HSC-3s (p < 0.05). NIR-PDT led to an increase in intracellular singlet oxygen and ROS (p < 0.05), and changes in mitochondrial membrane potential and apoptosis occurred (p < 0.01). At the same time, the permeability of the cell membrane increased over time (p < 0.05). These results suggest that this treatment can inhibit the proliferation of oral cancer cells and induce apoptosis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第977号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.18905/jodu.58.1_125","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Osaka Dental University. 2024, 58(1), p. 125-134."}]}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2024 The Author(s)","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 晋豪","creatorNameLang":"ja"},{"creatorName":"サイ, シンゴウ","creatorNameLang":"ja-Kana"},{"creatorName":"Cui, Jinhao","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-01"}],"filename":"kou_977_ab.pdf","filesize":[{"value":"256 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000089/files/kou_977_ab.pdf"},"version_id":"72e573c0-7b1a-445f-87cd-afa212f0251e"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-31"}],"filename":"kou_977_txt.pdf","filesize":[{"value":"602.1 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"学位論文","objectType":"fulltext","url":"https://osaka-dent.repo.nii.ac.jp/record/2000089/files/kou_977_txt.pdf"},"version_id":"167c7ab1-733f-47fe-8ebe-b50749f59b49"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Photodynamic therapy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oral squamous cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell membrane permeability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effectiveness of near-infrared light photodynamic therapy on oral cancer cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effectiveness of near-infrared light photodynamic therapy on oral cancer cells","subitem_title_language":"en"},{"subitem_title":"口腔癌細胞に対する近赤外光光線力学療法の有効性","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["65"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-01"},"publish_date":"2024-06-01","publish_status":"0","recid":"2000089","relation_version_is_last":true,"title":["Effectiveness of near-infrared light photodynamic therapy on oral cancer cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-02-02T07:25:46.566159+00:00"}