{"created":"2024-06-03T11:38:13.096391+00:00","id":2000106,"links":{},"metadata":{"_buckets":{"deposit":"acdbcb88-682e-4439-816f-800291d19c6a"},"_deposit":{"created_by":18,"id":"2000106","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000106"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000106","sets":["1686642104765:1:63:65"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-03-01"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for PCR in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes: actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第994号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.4012/dmj.2023-242","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Dental Materials Journal. 2024, 43(2), p. 172-178."}]}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2024 The Japanese Society for Dental Materials and Devices","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三好, 伸典","creatorNameLang":"ja"},{"creatorName":"ミヨシ, シンスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyoshi, Shinsuke","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-01"}],"filename":"kou_994_ab.pdf","filesize":[{"value":"260 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000106/files/kou_994_ab.pdf"},"version_id":"d5f5d499-0ffc-4236-bd21-496fe282e483"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-31"}],"filename":"kou_994_txt.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"学位論文","objectType":"fulltext","url":"https://osaka-dent.repo.nii.ac.jp/record/2000106/files/kou_994_txt.pdf"},"version_id":"cbccc4d3-5aea-414c-ba9f-672466b7d08d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Vascular aging, Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Reference gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Human whole saliva","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva","subitem_title_language":"en"},{"subitem_title":"血管老化診断キット開発におけるヒト唾液中参照遺伝子の探索","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["65"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-01"},"publish_date":"2024-06-01","publish_status":"0","recid":"2000106","relation_version_is_last":true,"title":["Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-02-02T07:27:33.001594+00:00"}