{"created":"2024-06-03T12:01:06.456201+00:00","id":2000111,"links":{},"metadata":{"_buckets":{"deposit":"e418db56-835a-4cdd-bd87-a2ede837d273"},"_deposit":{"created_by":18,"id":"2000111","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000111"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000111","sets":["1686642104765:1:63:65"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-03-01"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Periodontitis is a chronic infectious disease that causes gingival atrophy and tooth loss via alveolar bone resorption. Fusobacterium periodonticum is a key species involved in chronic periodontitis. Controlling the abundance of F. periodonticum is vital for the prevention and treatment of periodontal diseases. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) exerts bactericidal effects against Pseudomonas aeruginosa and Staphylococcus aureus. Here, we investigated the bactericidal potential of 5-ALA combined with PDT (ALA-PDT) against F. periodonticum. We used F. periodonticum JCM12991 and a 410-nm light-emitting diode in this study. First, we evaluated the bactericidal effect of ALA-PDT on F. periodonticum, and then explored its underlying mechanisms by analyzing its fluorescence, enzymatic activity, and membrane integrity. Culturing with 0.5% 5-ALA for 20 h confirmed a significant bactericidal effect of ALA-PDT against F. periodonticum. Fluorescence analysis revealed that F. periodonticum produced excitable photosensitive substances after the addition of 5-ALA. Furthermore, cell membrane disruption was observed in F. periodonticum following ALA-PDT. We found that ALA-PDT exerted antibacterial activity against F. periodonticum. If further studies confirm these results, this protocol could be an effective strategy for reducing the prevalence of periodontitis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第999号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.18905/jodu.58.1_219","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Osaka Dental University. 2024, 58(1), p. 219-226."}]}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2024 The Author(s)","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"王, 超","creatorNameLang":"ja"},{"creatorName":"オウ, チョウ","creatorNameLang":"ja-Kana"},{"creatorName":"Wang, Chao","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-01"}],"filename":"kou_999_ab.pdf","filesize":[{"value":"214 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000111/files/kou_999_ab.pdf"},"version_id":"7d37d924-acf5-489c-81df-d0ebf639490f"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-31"}],"filename":"kou_999_txt.pdf","filesize":[{"value":"473.1 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"学位論文","objectType":"fulltext","url":"https://osaka-dent.repo.nii.ac.jp/record/2000111/files/kou_999_txt.pdf"},"version_id":"1d1374d6-6d1d-4165-a408-e541fb8a4365"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fusobacterium periodonticum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"5-aminolevulinic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Photodynamic therapy","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effect of 5-aminolevulinic acid-mediated photodynamic therapy on  Fusobacterium periodonticum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of 5-aminolevulinic acid-mediated photodynamic therapy on  Fusobacterium periodonticum","subitem_title_language":"en"},{"subitem_title":"フソバクテリウム・ペリオドンティクムに対する5-アミノレブリン酸媒介光力学療法の殺菌効果","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["65"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-01"},"publish_date":"2024-06-01","publish_status":"0","recid":"2000111","relation_version_is_last":true,"title":["Effect of 5-aminolevulinic acid-mediated photodynamic therapy on  Fusobacterium periodonticum"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-02-02T07:28:07.112706+00:00"}