{"created":"2024-06-03T12:13:08.390106+00:00","id":2000114,"links":{},"metadata":{"_buckets":{"deposit":"e08d7249-8516-48a0-a9ef-8542b7bfd908"},"_deposit":{"created_by":18,"id":"2000114","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000114"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000114","sets":["1686642104765:1:63:65"]},"author_link":[],"control_number":"2000114","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-03-01"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Chronic periodontitis is a multifactorial disease that is strongly impacted by the oral microflora. Althought changes in the oral flora are related to the induction of gingival inflamation and alveolar bone resorption, there changes have not been extensively evaluated over time in patients with chronic periodontitis. Therefore, we analyzed the changes in the chronic periodontitis bacterial flora over time using next-generation sequencing. Subgingival plaque and saliva samples were collected from four patients diagnosed withchronic peridontitis at the time of the initial examination and at the end of basic periodontal treatment. The 16S rRNA gene region was amplified using PCR with DNA extracted from the samples as a template and sequenced using a next-generation sequencer. Bacterial flora were identified using QIIME2. Bacteria in the genera Streptococcus, Porphyromonas, Fusobacterium, Veillonella, Neisseria, Prevotella, Tannerella, and Corynebacterium were detected in 16 samples isolated from four participants. Alpha and beta diversity significantly differd between the four participants in the plaque and saliva flora, with no significant changes observed before and after treatment. A declining trend in Porphyromonas gingivalis and Fusobacterium nucleatum was observed exclusively in samples exhibiting a clinical response to the treatment.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1002号"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森, 祥太郎","creatorNameLang":"ja"},{"creatorName":"モリ, ショウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Mori, Shotaro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-01"}],"filename":"kou_1002_ab.pdf","filesize":[{"value":"190 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"本文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000114/files/kou_1002_ab.pdf"},"version_id":"b394a219-9a4f-41a5-bff0-9dbb8f8176bf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chronic periodontitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oral microflora","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"16S rRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"QIIME2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Longitudinal analysis of periodontitis bacterial flora by next-generation sequencing","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Longitudinal analysis of periodontitis bacterial flora by next-generation sequencing","subitem_title_language":"en"},{"subitem_title":"次世代シークエンサーを用いた慢性歯周炎細菌叢の経時解析","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["65"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-01"},"publish_date":"2024-06-01","publish_status":"0","recid":"2000114","relation_version_is_last":true,"title":["Longitudinal analysis of periodontitis bacterial flora by next-generation sequencing"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-06-07T07:00:56.558206+00:00"}