{"created":"2025-01-08T23:54:53.785289+00:00","id":2000166,"links":{},"metadata":{"_buckets":{"deposit":"32e6834e-76f1-430d-ab8d-68e9df7647e1"},"_deposit":{"created_by":18,"id":"2000166","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000166"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000166","sets":["1686642104765:1:1724813534049:1724813725424"]},"author_link":[],"control_number":"2000166","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-09-25"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Objectives\nRothia spp. are emerging as significant bacteria associated with oral health, with Rothia dentocariosa being one of the most prevalent species. However, there is a lack of studies examining these properties at the genetic level. This study aimed to establish a genetic modification platform for R. dentocariosa.\n\nMethods\nRothia spp. were isolated from saliva samples collected from healthy volunteers. Subsequently, R. dentocariosa strains were identified through colony morphology, species-specific polymerase chain reaction (PCR), and 16S ribosomal RNA gene sequencing. The identified strains were then transformed with plasmid pJRD215, and the most efficient strain was selected. Transposon insertion mutagenesis was performed to investigate the possibility of genetic modifications.\n\nResults\nA strain demonstrating high transforming ability, designated as R. dentocariosa LX16, was identified. This strain underwent transposon insertion mutagenesis and was screened for 5-fluoroorotic acid-resistant transposants. The insertion sites were confirmed using arbitrary primed PCR, gene-specific PCR, and Sanger sequencing.\n\nConclusion\nThis study marks the first successful genetic modification of R. dentocariosa. Investigating R. dentocariosa at the genetic level can provide insights into its role within the oral microbiome.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1011号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Journal of Oral Biosciences. 2024, 66(2), p. 358-364."}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.job.2024.04.006","subitem_relation_type_select":"DOI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2024 Japanese Association for Oral Biology","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"劉, 柏昂","creatorNameLang":"ja"},{"creatorName":"リュウ, ハクコウ","creatorNameLang":"ja-Kana"},{"creatorName":"Liu, Boang","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-12-23"}],"filename":"kou_1011_ab.pdf","filesize":[{"value":"225 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000166/files/kou_1011_ab.pdf"},"version_id":"b50d1b4c-13cd-45a0-ad21-9fa08e0d44cb"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2025-06-11"}],"filename":"kou_1011_txt.pdf","filesize":[{"value":"918 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"学位論文","objectType":"fulltext","url":"https://osaka-dent.repo.nii.ac.jp/record/2000166/files/kou_1011_txt.pdf"},"version_id":"84eb7257-04ac-4286-b3a2-118b6dc488d3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Rothia dentocariosa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Genetic modification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transposon insertion mutagenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Transposon insertion in Rothia dentocariosa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transposon insertion in Rothia dentocariosa","subitem_title_language":"en"},{"subitem_title":"Rothia dentocariosaに対するトランスポゾンの挿入","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["1724813725424"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-12-23"},"publish_date":"2024-12-23","publish_status":"0","recid":"2000166","relation_version_is_last":true,"title":["Transposon insertion in Rothia dentocariosa"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-07-10T04:10:00.141105+00:00"}