{"created":"2025-04-21T03:00:26.706740+00:00","id":2000170,"links":{},"metadata":{"_buckets":{"deposit":"0c761ff0-3b00-47e2-b7c6-785d5f989011"},"_deposit":{"created_by":18,"id":"2000170","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000170"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000170","sets":["1686642104765:1:1724813534049:1724813725424"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2025-03-07"}]},"item_10006_degree_grantor_9":{"attribute_name":"item_10006_degree_grantor_9","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"\"Aim: Ascorbic acid (AA) is a water-soluble vitamin that has antioxidant properties and regulates homeostasis of connective tissue through controlling various enzymatic activities. Two cell surface glycoproteins, sodium-dependent vitamin C transporter (SVCT) 1 and SVCT2, are known as ascorbate transporters. The purpose of this study was to investigate the expression pattern and functions of SVCTs in periodontal ligament (PDL) and PDL fibroblast (PDLF).\nMethods: Gene expression was examined using real-time polymerase chain reaction (PCR) and reverse transcription PCR. SVCT2 expression was determined by immunofluorescence staining, western blot and flow cytometry. ALP activity and collagen production were examined using ALP staining and collagen staining. Short interfering RNA was used to knock down the gene level of SVCT2. Change of comprehensive gene expression under SVCT2 knockdown condition was examined by RNA-sequencing analysis.\nResults: Real-time PCR, fluorescent immunostaining, western blot and flowy cytometry showed that SVCT2 was expressed in PDLF and PDL. ALP activity, collagen production, and SVCT2 expression were enhanced upon AA stimulation in PDLF. The enhancement of ALP activity, collagen production, and SVCT2 expression by AA was abolished under SVCT2 knockdown condition. RNA-sequencing revealed that gene expression of CLDN4, Cyclin E2, CAMK4, MSH5, DMC1, and Nidgen2 were changed by SVCT2 knockdown. Among them, the expression of MSH5 and DMC1, which are related to DNA damage sensor activity, was enhanced by AA, suggesting the new molecular target of AA in PDLF.\nConclusion: Our study reveals the SVCT2 expression in PDL and the pivotal role of SVCT2 in mediating AA-induced enhancements of ALP activity and collagen production in PDLF. Additionally, we identify alterations in gene expression profiles, highlighting potential molecular targets influenced by AA through SVCT2. These findings deepen our understanding of periodontal tissue homeostasis mechanisms and suggest promising intervention targeting AA metabolism.\"","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1012号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/jre.13322","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Periodontal Research. 2024, Online ahead of print."}]}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"神田, 智子","creatorNameLang":"ja"},{"creatorName":"カンダ, トモコ","creatorNameLang":"ja-Kana"},{"creatorName":"Tomoko, Kanda","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-04-21"}],"filename":"kou_1012_ab.pdf","filesize":[{"value":"269 KB"}],"format":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000170/files/kou_1012_ab.pdf"},"version_id":"fc8d056e-08ea-40f4-8b1d-0d1bfc514ed5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ALP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA damage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SVCT2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ascorbic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"collagen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontal ligament","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Role of sodium-dependent vitamin C transporter 2 in human periodontal ligament fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of sodium-dependent vitamin C transporter 2 in human periodontal ligament fibroblasts","subitem_title_language":"en"},{"subitem_title":"歯根膜線維芽細胞におけるsodium-dependent vitamin C transporter2の役割","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["1724813725424"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-04-21"},"publish_date":"2025-04-21","publish_status":"0","recid":"2000170","relation_version_is_last":true,"title":["Role of sodium-dependent vitamin C transporter 2 in human periodontal ligament fibroblasts"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-04-21T04:17:33.266208+00:00"}