{"created":"2025-04-25T05:58:50.705123+00:00","id":2000173,"links":{},"metadata":{"_buckets":{"deposit":"302eb643-48d7-464d-b8e6-eb30087e554b"},"_deposit":{"created_by":18,"id":"2000173","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000173"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000173","sets":["1686642104765:1:1724813534049:1724813725424"]},"author_link":[],"control_number":"2000173","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2025-03-07"}]},"item_10006_degree_grantor_9":{"attribute_name":"item_10006_degree_grantor_9","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The high-glucose conditions caused by diabetes mellitus (DM) exert several effects on cells, including inflammation. miR-146a, a kind of miRNA, is involved in inflammation and may be regulated mutually with reactive oxygen species (ROS), which are produced under high-glucose conditions. In the present study, we used human periodontal ligament cells (hPDLCs) to determine\n the effects of the high-glucose conditions of miR-146a and their involvement in the regulation of oxidative stress and inflammatory cytokines using Western blotting, PCR, ELISA and other methods. When hPDLCs were subjected to high glucose (24 mM), cell proliferation was not affected; inflammatory cytokine expression, ROS induction, interleukin-1 receptor-associated kinase 1 (IRAK1) and TNFreceptor-associated factor 6 (TRAF6) expression increased, but miR-146a expression decreased. Inhibition of ROS induction with the antioxidant N-acetyl-L-cysteine restored miR-146a expression and decreased inflammatory cytokine expression compared to those under high-glucose conditions. In addition, overexpression of miR-146a significantly suppressed the expression of the inflammatory cytokines IRAK1 and TRAF6, regardless of the glucose condition. Our findings suggest that oxidative stress and miR-146a expression are mutually regulated in hPDLCs under high-glucose conditions.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1015号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"International Journal of Molecular Sciences, 2024,25,19, 10702"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms251910702","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"文元, 智優","creatorNameLang":"ja"},{"creatorName":"フミモト, チヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Chihiro, Fumimoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"山内, 伸浩","creatorNameLang":"ja"},{"creatorName":"ヤマウチ, ノブヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Nobuhiro, Yamauchi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"皆川, 咲佳","creatorNameLang":"ja"},{"creatorName":"ミナガワ, エミカ","creatorNameLang":"ja-Kana"},{"creatorName":"Emika, Minagawa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"梅田, 誠","creatorNameLang":"ja"},{"creatorName":"ウメダ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Makoto, Umeda","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-04-25"}],"filename":"kou_1015_ab.pdf","filesize":[{"value":"234 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000173/files/kou_1015_ab.pdf"},"version_id":"14ac2bb8-1b4e-4b28-be6a-8a2b37514cf3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"miR-146a","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hPDLCs","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ROS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high Glucose","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"MiR-146a Is Mutually Regulated by High Glucose Induced  Oxidative Stress in Human Periodontal Ligament Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MiR-146a Is Mutually Regulated by High Glucose Induced  Oxidative Stress in Human Periodontal Ligament Cells","subitem_title_language":"en"},{"subitem_title":"ヒト歯根膜細胞における高血糖の酸化ストレスと miR-146a発現の相互制御","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["1724813725424"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-04-25"},"publish_date":"2025-04-25","publish_status":"0","recid":"2000173","relation_version_is_last":true,"title":["MiR-146a Is Mutually Regulated by High Glucose Induced  Oxidative Stress in Human Periodontal Ligament Cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-04-30T05:44:46.320033+00:00"}