{"created":"2025-05-26T06:29:25.657797+00:00","id":2000194,"links":{},"metadata":{"_buckets":{"deposit":"dda26537-76bb-4352-a587-a8c7c1e9f774"},"_deposit":{"created_by":18,"id":"2000194","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000194"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:02000194","sets":["1686642104765:1:1724813534049:1724813725424"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2025-03-07"}]},"item_10006_degree_grantor_9":{"attribute_name":"item_10006_degree_grantor_9","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Fusobacterium nucleatum (F. nucleatum), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic  therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets F. nucleatum by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on F. nucleatum has not been  evaluated previously. This study investigates the effect of ALA-PDT using red light-emitting diode (LED) light on F. nucleatum subspecies and their porphyrin accumulation. F. nucleatum subspecies were cultured with varying  concentrations of 5-ALA under anaerobic conditions. Porphyrin accumulation was measured via fluorescence spectroscopy, and colony-forming units were measured to determine bacterial viability post-treatment. Additionally, other  subspecies responded well to 0.01% 5-ALA, and uroporphyrin I accumulation correlated with bacterial death, revealing optimal bactericidal conditions. These results suggest that optimizing light intensity and 5-ALA concentration can  significantly enhance the therapeutic potential of ALA-PDT in oral healthcare.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1035号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Pathogens, 2024, 13(11), p. 1016"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/pathogens13111016","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"李, 江嵐","creatorNameLang":"ja"},{"creatorName":"リ, コウラン","creatorNameLang":"ja-Kana"},{"creatorName":"Li, Jianglan","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-05-26"}],"filename":"kou_1035_ab.pdf","filesize":[{"value":"249 KB"}],"format":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/2000194/files/kou_1035_ab.pdf"},"version_id":"07237d57-d171-46d9-9405-68d29d799c91"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"5-aminolevulinic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fusobacterium nucleatum","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"photodynamic therapy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"red light-emitting diode","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"subspecies","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against Fusobacterium nucleatum Subspecies","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against Fusobacterium nucleatum Subspecies","subitem_title_language":"en"},{"subitem_title":"Fusobacterium nucleatum 亜種に対して効果的な殺菌作用を有する赤色光を使用した最適な光線力学療法","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"18","path":["1724813725424"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-05-26"},"publish_date":"2025-05-26","publish_status":"0","recid":"2000194","relation_version_is_last":true,"title":["Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against Fusobacterium nucleatum Subspecies"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-05-26T06:39:12.317347+00:00"}