{"created":"2023-05-15T13:57:36.756542+00:00","id":320,"links":{},"metadata":{"_buckets":{"deposit":"f82991c3-1adf-48db-94e6-1a2673621ecc"},"_deposit":{"created_by":16,"id":"320","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"320"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000320","sets":["1686642104765:1:53:56"]},"author_link":["809","808"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ラット先天性裂顎モデルにおける新規細胞移植プラットフォーム CellSaic を使用した歯髄および骨髄間葉系幹細胞の骨形成能の比較"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-04"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2021年度","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Scaffolds stimulate cell proliferation and differentiation and play major roles in providing growth and nutrition factors in the repair of bone defects. We used the recombinant peptide Cellnest™to prepare the three-dimensional stem cell complex, CellSaic, and evaluated whether CellSaic con taining rat dental pulp stem cells (rDPSCs) was better than that containing rat bone marrow stem cells (rBMSCs). rDPSC-CellSaic or rBMSC-CellSaic, cultured with or without osteogenic induction medium, formed the experimental and control groups, respectively. Osteoblast differentiation was evaluated in vitro and transplanted into a rat model with a congenital jaw fracture. Specimens were collected and evaluated by microradiology and histological analysis. In the experimental group, the amount of calcium deposits, expression levels of bone-related genes (RUNX2, ALP, BSP, and COL1),and volume of mineralized tissue, were significantly higher than those in the control group (p < 0.05).Both differentiated and undifferentiated rDPSC-CellSaic and only the differentiated rBMSC-CellSaic could induce the formation of new bone tissue. Overall, rBMSC-CellSaic and rDPSC-CellSaic made with Cellnest™ as a scaffold, provide excellent support for promoting bone regeneration in rat mandibular congenital defects. Additionally, rDPSC-CellSaic seems a better source for craniofacial bone defect repair than rBMSC-CellSaic, suggesting the possibility of using DPSCs in bone tissue regenerative therapy","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第922号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"呂, 金釗"},{"creatorName":"ロ, キンショウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"808","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lyu, JinZhao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"809","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-13"}],"displaytype":"detail","filename":"kou_922_txt_最適化.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/320/files/kou_922_txt_最適化.pdf"},"version_id":"f1b835f8-4f71-4b5f-ac12-3b2a8fc4541e"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-13"}],"displaytype":"detail","filename":"kou_922.pdf","filesize":[{"value":"227.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/320/files/kou_922.pdf"},"version_id":"a0943902-2e17-4f66-a7dd-cbf272e2c5d0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"stem cells｜Cellnest™｜CellSaic｜ rDPSCs｜rBMSCs｜regeneration｜osteogenic differentiation｜bone repair｜rat congenital cleft-jaw model","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model"}]},"item_type_id":"10006","owner":"16","path":["56"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-13"},"publish_date":"2022-05-13","publish_status":"0","recid":"320","relation_version_is_last":true,"title":["Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-05-15T14:06:17.513395+00:00"}