{"created":"2023-05-15T13:57:39.324024+00:00","id":366,"links":{},"metadata":{"_buckets":{"deposit":"f4995a5b-228a-4333-9a41-2f23daf26b8f"},"_deposit":{"created_by":16,"id":"366","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"366"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000366","sets":["1686642104765:1:57:60"]},"author_link":["900"],"control_number":"366","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-03-03"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Certain siderophores have recently been reported to inhibit the growth of cancerous cells. Here, we assessed the cytotoxic effects of two siderophores, deferriferrichrysin (Dfcy) and bisucaberin, on the human oral squamous cell carcinoma (OSCC) cell lines HSC-3 and SAS. The iron-chelating ability of Dfcy and bisucaberin was measured using an iron assay kit utilizing the ferrozine chromogenic method. Cell viability was measured using the WST-8 assay, and caspase-3 activity was determined using a caspase-3 assay kit. Apoptotic, anti-apoptotic, and cell cycle markers were assessed by western blot analysis. Dfcy chelated ferric iron in a dose-dependent manner. In addition, 10 µg/mL bisucaberin chelated approximately 75% of the iron. Dfcy and bisucaberin inhibited the proliferation of HSC-3 and SAS cells in a dose-dependent manner. Bisucaberin-treated SAS cells exhibited significantly increased caspase-3 activation. Cleaved poly(ADP-ribose) polymerase (PARP) was also increased in bisucaberin-treated HSC-3 and SAS cells and in Dfcy-treated SAS cells.  Bisucaberin treatments alone upregulated cleaved caspase-3 in SAS cells. However, Dfcy had no effect on OSCC cell apoptosis. The results show that bisucaberin exerts potential anticancer effects against SAS cells by inducing apoptosis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第958号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.18905/jodu.57.1_89","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Osaka Dental University. 2023, 57(1), p. 89-97."}]}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2023 The Author(s)","subitem_rights_language":"en"}]},"item_1696310207136":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷, 暢","creatorNameLang":"ja"},{"creatorName":"タニ, ミツル","creatorNameLang":"ja-Kana"},{"creatorName":"Tani, Mitsuru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"900","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-04-04"}],"filename":"kou_958.pdf","filesize":[{"value":"261.7 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/366/files/kou_958.pdf"},"version_id":"01bb0a81-7f91-49da-af08-57a1393f4206"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-03-01"}],"filename":"kou_958_fulltxt.pdf","filesize":[{"value":"804 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"学位論文","objectType":"fulltext","url":"https://osaka-dent.repo.nii.ac.jp/record/366/files/kou_958_fulltxt.pdf"},"version_id":"27803662-c2c1-44ac-8fad-721eb5780a14"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Deferriferrichrysin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bisucaberin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Siderophore","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oral squamous cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effect of cell death induced by deferriferrichrysin on oral squamous cell carcinoma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of cell death induced by deferriferrichrysin on oral squamous cell carcinoma cells","subitem_title_language":"en"},{"subitem_title":"口腔扁平上皮癌細胞におけるdeferriferrichrysinの細胞死誘導効果","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"16","path":["60"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-04"},"publish_date":"2023-04-04","publish_status":"0","recid":"366","relation_version_is_last":true,"title":["Effect of cell death induced by deferriferrichrysin on oral squamous cell carcinoma cells"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2024-03-13T03:54:49.312081+00:00"}