{"created":"2023-05-15T13:57:39.861288+00:00","id":377,"links":{},"metadata":{"_buckets":{"deposit":"6cafcc1c-37de-4f67-a4d8-a4c1186439d3"},"_deposit":{"created_by":16,"id":"377","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"377"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000377","sets":["1686642104765:1:57:60"]},"author_link":["922"],"control_number":"377","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-03-03"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"大阪歯科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Osaka Dental University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Early osseointegration is important for achieving initial stability after implant placement, and our collaborators used atmospheric pressure plasma treatment as one of the methods to make the titanium surface super hydrophilic. According to the researchers, this was useful for improving the initial adhesion of rat bone-marrow cells and their ability to induce hard-tissue differentiation without changing the properties of the material surface. Therefore, in this study, we compared and investigated the effects of atmospheric pressure plasma treatment of titanium surface on the behavior of rat vascular endothelial cells (ECs). The experimental material was a JIS grade 2 titanium surface. The untreated surface served as the control group, and the one irradiated with atmospheric pressure plasma via a piezo  brush  served  as  the  experimental  group. X-ray photoelectron spectroscopy (XPS) was used to analyze the elemental composition of the sample surface. Furthermore, the contact angle of distilled water on the surface of each group was 3measured. Subsequently, ECs were isolated from the descending thoracic aorta of 8-week-old male SD rats and cultured in primary cultures. The number of cell adhesions between the experimental and control groups and the expression of genes related to the initial adhesion of ECs were measured 2 and 5 days after the culture began. Student’s t-test was used for statistical analysis on various measured values, with the significance level set to 5% or less. In the experimental group, XPS observations revealed a decrease in carbon peak intensity and hydroxide formation. When the cell adhesion numbers were compared, the experimental group exhibited significantly higher values at all measurement times. Furthermore, the experimental group exhibited significantly higher gene expression values, indicating an initial adhesion to the material surface at all measurement times. When compared to the control group, fluorescent microscopic images revealed that the experimental group had longer and more widely attached cell processes. These findings indicate that atmospheric-pressure plasma treatment of titanium is beneficial for improving EC adhesion and gene expression of adhesion factors.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第969号"}]},"item_10006_relation_14":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"日本口腔リハビリテーション学会雑誌. 2022, 35(1), p. 36-47."}]}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 The Japan Association of Oral Rehabilitation","subitem_rights_language":"en"}]},"item_1696310207136":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田﨑, 智香子","creatorNameLang":"ja"},{"creatorName":"タサキ, チカコ","creatorNameLang":"ja-Kana"},{"creatorName":"Tasaki, Chikako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"922","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-04-05"}],"filename":"kou_969.pdf","filesize":[{"value":"232.6 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","objectType":"abstract","url":"https://osaka-dent.repo.nii.ac.jp/record/377/files/kou_969.pdf"},"version_id":"40fa90a8-41bd-4d97-a3a8-942f347bb8a6"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-03-01"}],"filename":"kou_969_fulltxt.pdf","filesize":[{"value":"656 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"学位論文","objectType":"fulltext","url":"https://osaka-dent.repo.nii.ac.jp/record/377/files/kou_969_fulltxt.pdf"},"version_id":"803101a8-e69d-40f5-a577-979fec795e2a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"implant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vasucular endothelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"atmospheric pressure plasma","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effect of atmospheric pressure plasma treatment on titanium surface on the initial behavior of vascular endothelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of atmospheric pressure plasma treatment on titanium surface on the initial behavior of vascular endothelial cells","subitem_title_language":"en"},{"subitem_title":"純チタン金属への大気圧プラズマ処理が血管内皮細胞の初期挙動に与える影響について","subitem_title_language":"ja"}]},"item_type_id":"10006","owner":"16","path":["60"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-05"},"publish_date":"2023-04-05","publish_status":"0","recid":"377","relation_version_is_last":true,"title":["Effect of atmospheric pressure plasma treatment on titanium surface on the initial behavior of vascular endothelial cells"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2024-03-13T03:54:45.951391+00:00"}