{"created":"2023-05-15T13:57:21.989607+00:00","id":43,"links":{},"metadata":{"_buckets":{"deposit":"1c562498-f8ce-43f1-b251-aed58a465d57"},"_deposit":{"created_by":15,"id":"43","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"43"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000043","sets":["1686642104765:1:7:10"]},"author_link":["125","126"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ネクロプトーシス、アポトーシス阻害の組み合わせは心筋虚血再灌流障害に対する心保護効果を増強する"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-07"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Necroptosis has been proposed as a mode of cell death that is a caspase-independent programmed necrosis. We investigated whether necroptosis is involved in myocardial ischemia-reperfusion injury in isolated guinea pig hearts and, if so, whether simultaneous inhibition of necroptosis and apoptosis confers enhanced cardioprotection.","subitem_description_type":"Abstract"},{"subitem_description":"Isolated perfused guinea pig hearts were subjected to 30 min ischemia and 4 h reperfusion (control = CTL, n = 8). Necrostatin-1 (necroptosis inhibitor, 10 μM), Z-VAD (apoptosis inhibitor, 0.1 μM) and both inhibitors were administered starting 5 min before ischemia and during the initial 30 min of reperfusion (Nec, Z-VAD, Nec + Z-VAD; n = 8 each). Contractile recovery was monitored by left ventricular developed (LVDP) and end-diastolic (LVEDP) pressure. Infarct size was determined by triphenyltetrazolium chloride staining. Tissue samples were obtained after 4 h reperfusion to determine expression of receptor-interacting protein 1 (RIP1) and activated caspase 3 by Western blot analysis.","subitem_description_type":"Abstract"},{"subitem_description":"After reperfusion, Nec + Z-VAD had higher LVDP and lower LVEDP compared with CTL. Infarct size was reduced in Nec and Z-VAD compared with CTL. Combination of necroptosis and apoptosis inhibition further reduced infarct size. Expression of activated caspase 3 was not increased in Z-VAD and Nec + Z-VAD compared with Nec and CTL. Expression of RIP1 was preserved in Z-VAD and Nec + Z-VAD compared with CTL, suggesting RIP1-mediated necrosis is involved in myocardial ischemia-reperfusion injury.","subitem_description_type":"Abstract"},{"subitem_description":"Necroptosis is involved in myocardial ischemia-reperfusion injury, and simultaneous inhibition of necroptosis and apoptosis enhances the cardioprotective effect. These findings may provide a novel, additive strategy for cardioprotection in acute myocardial infarction.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第719号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"越沼, 静"},{"creatorName":"コシヌマ, シズカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"126","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koshinuma, Shizuka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"125","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"kou_719_txt..pdf","filesize":[{"value":"442.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/43/files/kou_719_txt..pdf"},"version_id":"66f89e5d-2929-4d18-822a-c5b46128241f"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"kou_719.pdf","filesize":[{"value":"127.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/43/files/kou_719.pdf"},"version_id":"732f200c-44b0-4c77-a91d-074ea2fc4382"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Amino Acid Chloromethyl Ketones","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cardiotonic Agents","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Caspase 3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Drug Therapy, Combination","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Guinea Pigs","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Heart","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hemodynamics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Imidazoles","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Indoles","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Male","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Myocardial Reperfusion Injury","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Myocardium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Necrosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury"}]},"item_type_id":"10006","owner":"15","path":["10"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-01"},"publish_date":"2016-08-01","publish_status":"0","recid":"43","relation_version_is_last":true,"title":["Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury"],"weko_creator_id":"15","weko_shared_id":15},"updated":"2023-05-15T14:09:42.654521+00:00"}