{"created":"2023-05-15T13:57:23.006904+00:00","id":62,"links":{},"metadata":{"_buckets":{"deposit":"d495bf7b-b00f-410f-92d8-13d99545a44c"},"_deposit":{"created_by":15,"id":"62","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"62"},"status":"published"},"_oai":{"id":"oai:osaka-dent.repo.nii.ac.jp:00000062","sets":["1686642104765:1:7:10"]},"author_link":["237","236"],"item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"LPSによるヒト歯根膜幹細胞の骨芽細胞分化能の阻害と炎症性サイトカインの産生"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-07"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"大阪歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34408","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective\nPorphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) induces pro-inflammatory cytokines, such as interleukin-1 β (IL-1β), IL-6, and IL-8, which induce periodontal tissue destruction. Periodontal ligament stem cells (PDLSCs) play an important role in periodontal tissue regeneration and are expected to have future applications in cellular therapies for periodontitis. However, no studies have examined the effects of P. gingivalis LPS on PDLSCs. The aim of this study was to investigate how P. gingivalis LPS affects the osteoblastic differentiation and pro-inflammatory cytokine production of PDLSCs.\nDesign\nPDLSCs were obtained from healthy adult human mandibular third molars. The identification of PDLSCs was confirmed by immunohistochemical evaluations of the mesenchymal stem cell markers STRO-1 and SSEA-4. Cell proliferation and osteoblastic differentiation were investigated by culturing the PDLSCs in a normal or osteogenic medium with P. gingivalis LPS (0, 1, or 10 μg/mL) and then measuring the alkaline phosphatase (ALP) activity and the production of collagen type 1 Alpha 1 (COL1A1), osteocalcin production, and mineralisation. Additionally, we examined the production of IL-1β, IL-6, and IL-8 in the PDLSCs.\nResults\nP. gingivalis LPS inhibited the ALP activity, COL1A1 and osteocalcin production, and mineralisation in the PDLSCs, which are positive for STRO-1 and SSEA-4. P. gingivalis LPS also promoted cell proliferation and produced IL-1β, IL-6, and IL-8.\nConclusions\nThis study provides the first findings that P. gingivalis LPS inhibits osteoblastic differentiation and induces pro-inflammatory cytokines in PDLSCs. These findings will help clarify the relationship between periodontitis and periodontal tissue regeneration.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第739号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"嘉藤, 弘仁"},{"creatorName":"カトウ, ヒロヒト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"236","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Hirohito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"237","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"kou_739_txt..pdf","filesize":[{"value":"723.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"学位論文","url":"https://osaka-dent.repo.nii.ac.jp/record/62/files/kou_739_txt..pdf"},"version_id":"39c4aff4-ea29-4290-9562-8022d0ceaecd"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-01"}],"displaytype":"detail","filename":"kou_739.pdf","filesize":[{"value":"120.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容要旨・審査結果要旨","url":"https://osaka-dent.repo.nii.ac.jp/record/62/files/kou_739.pdf"},"version_id":"00f5e595-58ed-43b2-8ec8-f9ea5cb95b67"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Periodontal ligament","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipopolysaccharides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytokines","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells"}]},"item_type_id":"10006","owner":"15","path":["10"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-08-01"},"publish_date":"2016-08-01","publish_status":"0","recid":"62","relation_version_is_last":true,"title":["Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells"],"weko_creator_id":"15","weko_shared_id":15},"updated":"2023-05-15T14:08:02.282188+00:00"}