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A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type Ⅰ-A collagen gel scaffold in vitro
https://osaka-dent.repo.nii.ac.jp/records/140
https://osaka-dent.repo.nii.ac.jp/records/14091eeb6ec-66cb-47cc-9ac7-df18284d48f1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
学位論文 (5.0 MB)
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論文内容要旨・審査結果要旨 (212.3 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-08-01 | |||||
| 本文言語 | ||||||
| 言語 | eng | |||||
| タイトル | ||||||
| タイトル | A histological study of mineralised tissue formation around implants with 3D culture of HMS0014 cells in Cellmatrix Type Ⅰ-A collagen gel scaffold in vitro | |||||
| 著者 |
森下, 愛子
× 森下, 愛子 |
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| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Turn over | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mesenchymal stem cells (MSCs) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 3D collagen scaffold | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | GBR tissue | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We cultured HMS0014 Yub621b cells within a 3D collagen gel scaffold (Cellmatrix Type I-A) and aimed to study the fate and contribution of human bone-derived mesenchymal stem cells (MSCs) in the guided bone regeneration(GBR)-engineered tissue which has developed around the titanium (Ti) test dental implant (IP) in vitro. The light microscopy (LM) and transmission electron microscopy (TEM) results of the peri-IP tissue indicated that collagen fibrils of the Cellmatrix Type I-A gel were accumulated and fabricated to provide a 3D meshwork for proliferation and differentiation of the HMS0014 cells in the top (cell) layer; mineralisation of the GBR tissue had commenced since day 1 and became markedly deposited between days 7 and 14 of the experiment. TEM observation revealed sedimentation of cement line at the periphery of the interwoven Cellmatrix fibres and fibrils in the ECM scaffold of the GBR tissue; matrix vesicle-mediated and appositional collagen-mediated mineralisation were identified in the peri-IP ECM scaffold. The fine structure study of the plurimorphic osteoblast(Ob)-like osteogeneic cells demonstrated numerous membranous organelles related with vesicular trafficking, secretion and endocytosis in the cytoplasm; well-developed cytoskeleton networks and intercellular junctional complexes were also observed. The specimens on fluorescence immunohistochemistry (IHC) by confocal laser-scanning microscopy (LSM) showed the expression of LC3 and Cx43 associated with autophagic-lysosomal degeneration pathway and signal conduction mediated with gap junctions (GJS) in maintaining tissue homeostasis of the Ob-like cells which grew and degenerated in the 3D scaffold. Results from this in vitro study suggest that Ob-like HMS0014 cells actively regulate turnover of the peri-IP ECM to recapitulate the development and formation of osteoid tissue-engineered material which might contribute to augment osseointegration around the dental implant. | |||||
| 学位名 | ||||||
| 学位名 | 博士（歯学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 34408 | |||||
| 学位授与機関名 | 大阪歯科大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2015-09-18 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 乙第1600号 | |||||
