| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2016-08-01 |
| 本文言語 |
|
|
言語 |
eng |
| タイトル |
|
|
タイトル |
Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury |
| 著者 |
越沼, 静
Koshinuma, Shizuka
|
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Amino Acid Chloromethyl Ketones |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Animals |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Apoptosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cardiotonic Agents |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Caspase 3 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Drug Therapy, Combination |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Guinea Pigs |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Heart |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hemodynamics |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Imidazoles |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Indoles |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Male |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Myocardial Reperfusion Injury |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Myocardium |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Necrosis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Necroptosis has been proposed as a mode of cell death that is a caspase-independent programmed necrosis. We investigated whether necroptosis is involved in myocardial ischemia-reperfusion injury in isolated guinea pig hearts and, if so, whether simultaneous inhibition of necroptosis and apoptosis confers enhanced cardioprotection. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Isolated perfused guinea pig hearts were subjected to 30 min ischemia and 4 h reperfusion (control = CTL, n = 8). Necrostatin-1 (necroptosis inhibitor, 10 μM), Z-VAD (apoptosis inhibitor, 0.1 μM) and both inhibitors were administered starting 5 min before ischemia and during the initial 30 min of reperfusion (Nec, Z-VAD, Nec + Z-VAD; n = 8 each). Contractile recovery was monitored by left ventricular developed (LVDP) and end-diastolic (LVEDP) pressure. Infarct size was determined by triphenyltetrazolium chloride staining. Tissue samples were obtained after 4 h reperfusion to determine expression of receptor-interacting protein 1 (RIP1) and activated caspase 3 by Western blot analysis. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
After reperfusion, Nec + Z-VAD had higher LVDP and lower LVEDP compared with CTL. Infarct size was reduced in Nec and Z-VAD compared with CTL. Combination of necroptosis and apoptosis inhibition further reduced infarct size. Expression of activated caspase 3 was not increased in Z-VAD and Nec + Z-VAD compared with Nec and CTL. Expression of RIP1 was preserved in Z-VAD and Nec + Z-VAD compared with CTL, suggesting RIP1-mediated necrosis is involved in myocardial ischemia-reperfusion injury. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Necroptosis is involved in myocardial ischemia-reperfusion injury, and simultaneous inhibition of necroptosis and apoptosis enhances the cardioprotective effect. These findings may provide a novel, additive strategy for cardioprotection in acute myocardial infarction. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与機関 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2014-03-07 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第719号 |
学位論文 (442.3 kB)
