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Expression of SHH, PTCH and SMO to the Hedgehog signal in keratocystic odontogenic tumor
https://osaka-dent.repo.nii.ac.jp/records/14
https://osaka-dent.repo.nii.ac.jp/records/149d75d8c1-14fe-4f3a-be07-d56ecad0e797
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (385.9 kB)
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論文内容要旨・審査結果要旨 (179.9 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2016-08-01 | |||||||||||
| 本文言語 | ||||||||||||
| 言語 | eng | |||||||||||
| タイトル | ||||||||||||
| タイトル | Expression of SHH, PTCH and SMO to the Hedgehog signal in keratocystic odontogenic tumor | |||||||||||
| 著者 |
⼭⽥, 康嗣
× ⼭⽥, 康嗣
× Yamada, Koji
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| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Keratocystic odontogenic tumor | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Sonic hedgehog | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Patched 1 protein | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Smoothened protein | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Keratocystic odontogenic tumor | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Sonic hedgehog | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Patched 1 protein | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Smoothened protein | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The keratocystic odontogenic tumor (KCOT) is a benign odontogenic tumor of the jaw that occurs in association with nevoid basal cell carcinoma syndrome (NBCCS). In 2005, WHO recommended that the term KCOT replace the term odontogenic keratocyst (OKC), because of the aggressive biological behavior of KCOT, and its expression of the Patched 1 (PTCH) gene that is related to KCOT tumorigenesis. We investigated the co-expression of PTCH with that of sonic hedgehog (SHH), for which PTCH is a receptor, and of smoothened (SMO), a molecule downstream of PTCH signaling to determine if this signaling pathway might be intact in KCOT. We used immunohistochemistry, western blotting and RT-PCR analyses to examine the expression of SHH, PTCH and SMO in surgical specimens of 30 cases of KCOT, 8 cases of the orthokeratinized odontogenic cyst (OOC) and 10 cases of the dentigerous cyst (DC). We also did immunohistochemical analyses of two different epithelial layers, the upper spinous layer and the lower basal layer. Immunohistochemical analysis revealed that the spinous layer of 28 cases and the basal layer of 15 cases were positive for SHH, the spinous layer of all cases and the basal layer of 23 cases were positive for PTCH, and the spinous layer of 28 cases and the basal layer of 15 cases were positive for SMO. Western blotting showed that SHH protein expression, with a molecular weight of 17 kDa, was detected in KCOT, OOC and DC ; a PTCH protein, with a molecular weight of 161 kDa, was observed in a specific pattern in KCOT, and an SMO protein, with a molecular weight of 46 kDa, was observed in KCOT. PCR analysis indicated a specific expression pattern of PTCH in KCOT, OOC and DC. Since PTCH, SHH and SMO were expressed in some cases of KCOT, we think that non-mutated molecules in the SHH pathway may be responsible for the development of KCOT, OOC and DC. | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 34408 | |||||||||||
| 学位授与機関名 | 大阪歯科大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2015-03-06 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第750号 | |||||||||||
