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Effects of NOD1 in Human Dental Pulp Fibroblast Like cells
https://osaka-dent.repo.nii.ac.jp/records/191
https://osaka-dent.repo.nii.ac.jp/records/191e6e88365-5ad4-4bb2-8ffd-f0944590d36b
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (435.2 kB)
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論文内容要旨・審査結果要旨 (210.0 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2018-05-17 | |||||||||
| 本文言語 | ||||||||||
| 言語 | eng | |||||||||
| タイトル | ||||||||||
| タイトル | Effects of NOD1 in Human Dental Pulp Fibroblast Like cells | |||||||||
| 著者 |
岩佐, 一弘
× 岩佐, 一弘
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| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Matrix metalloproteinase-3 | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | NOD1 | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | iE-DAP | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | JNK | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | RIP2 | |||||||||
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| 主題Scheme | Other | |||||||||
| 主題 | Human pulp fibroblasts | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Purpose: Caries-related immune response is first recognized by dental pulp fibroblast cells and is tightly regulated to avert pulpitis. Nucleotide-binding oligomerization domain (NOD) 1 signaling, which is essential for initiating the innate immune response to bacterial infection, is subject to many regulatory mechanisms. However, little is known about post transcriptional regulation of NOD1 dependent responses in dental pulp fibroblast cells. NOD1 recognizes D-glutamyl-meso-diaminopimelic acid (iE-DAP). RIP2 is associated with NOD1 and play critical roles in the immune system. Matrix metalloproteinases (MMPs) such as MMP-1, 2, 3, and 14 were also shown to be expressed in inflamed dental pulp tissue. MMP-3 can degrade the extracellular matrix (ECM) and activate other MMPs. MMP-3 is considered to be involved in wound healing, inflammation, and tumor initiation. Dental pulp destruction may be regulated, in part, by matrix metalloproteinase-3 (MMP-3), and other MMPs activated by MMP-3 have been shown to regulate the degradation and regeneration of dental pulp tissue. In the present study, we investigated that MMP-3 was produced in response to iE-DAP and cell signaling in human pulp fibroblasts (HPFs). Methods: HPFs were incubated in serum-free α-MEM containing iE-DAP (10 g/mL) for 24 h with or without the RIP inhibitor, 0.5, 1, 5, 10, 15, 20 nM Gefitinib. The production of MMP-3 and expression of RIP2 were evaluated by RIP2 and MMP-3 antibodies using western blot analysis. HPFs were incubated in serum-free α-MEM containing iE-DAP (10 g/mL) for 24 h with or without the JNK inhibitor, AS601245 or SP600125. The production of MMP-3 and activation of JNK by iE-DAP were evaluated by the phosphorylation of JNK and MMP-3 antibodies using western blot analysis. Results: iE-DAP enhanced the production of MMP-3 in a dose dependent manner in HPFs. RIP2 inhibitor suppressed the production of MMP-3 on iE-DAP stimulated HPFs. We demonstrated that MMP-3 was produced from HPFs in response to iE-DAP in a JNK-dependent manner. Conclusion: These results suggest that iE-DAP/NOD1 induced the production of MMP-3 in HPFs through a signaling cascade involving RIP2-mediated phosphorylation of JNK. |
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| 学位名 | ||||||||||
| 学位名 | 博士（歯学） | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 34408 | |||||||||
| 学位授与機関名 | 大阪歯科大学 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2018-03-09 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第829号 | |||||||||
