| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2019-05-17 |
| 本文言語 |
|
|
言語 |
eng |
| タイトル |
|
|
タイトル |
Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model |
| 著者 |
笹山, 智史
Sasayama, Satoshi
|
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
plant polyphenol｜ EGCG｜ gelatin｜bone formation｜congenital bone defect｜dedifferentiated fat cell｜adipose-derived stem cell｜scaffold |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofacial abnormalities and injuries. One potential strategy is to utilize pharmacological and cost-effective plant polyphenols and biocompatible proteins, such as gelatin. Nevertheless, the use of chemically modified proteins with plant polyphenols in this strategy has not been standardized. Here, we demonstrated that gelatin chemically modified with epigallocatechin gallate (EGCG), the major catechin isolated from green tea, can be a useful material to induce bone regeneration in a rat congenial cleft-jaw model in vivo when used with/without adipose-derived stem cells or dedifferentiated fat cells. Vacuum-heated gelatin sponges modified with EGCG (vhEGCG-GS) induced superior osteogenesis from these two cell types compared with vacuum-heated gelatin sponges (vhGS). The EGCG-modification converted the water wettability of vhGS to a hydrophilic property (contact angle: 110° to 3.8°) and the zeta potential to a negative surface charge; the modification enhanced the cell adhesion property and promoted calcium phosphate precipitation. These results suggest that the EGCG-modification with chemical synthesis can be a useful platform to modify the physicochemical property of gelatin. This alteration is likely to provide a preferable microenvironment for multipotent progenitor cells, inducing superior bone formation in vivo. |
| 学位名 |
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|
学位名 |
博士（歯学） |
| 学位授与機関 |
|
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学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
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学位授与年月日 |
2019-03-08 |
| 学位授与番号 |
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学位授与番号 |
甲第838号 |
学位論文 (4.3 MB)
