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Gene analysis of ameloblastoma-derived cells treated with retinoic acid
https://osaka-dent.repo.nii.ac.jp/records/258
https://osaka-dent.repo.nii.ac.jp/records/258f9934160-593d-4807-b6ff-3c157d31a369
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (430.2 kB)
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論文内容要旨・審査結果要旨 (227.9 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2020-04-28 | |||||||||||
| 本文言語 | ||||||||||||
| 言語 | eng | |||||||||||
| タイトル | ||||||||||||
| タイトル | Gene analysis of ameloblastoma-derived cells treated with retinoic acid | |||||||||||
| 著者 |
宮, 由紀子
× 宮, 由紀子
× Miya, Yukiko
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| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Ameloblastoma｜Retinoic acid ｜Microarray ｜Cultured cells | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Ameloblastoma is one of the most common odontogenic benign tumors in the jawbone, and may recur because it grows in a locally invasive manner. Although many studies have been performed on ameloblastoma, its bioactive characteristics remain unclear. Retinoic acid, a metabolite of fat-soluble vitamin A used as a leukemia drug, has been demonstrated to have antitumor effects on some tumor types. The present study examined whether retinoic acid has potential as a new therapeutic drug for ameloblastoma. We found that retinoic acid 1) inhibited the proliferation of cells derived from ameloblastoma and 2) induced differentiation. However, its intracellular signaling and regulatory mechanisms remain unclear. In this study, we primarily cultured cells from ameloblastoma to elucidate signal transduction, and examined the differences in gene expression levels due to retinoic acid action by microarray analysis. Surgically removed specimens were obtained with consent from the Second Department of Oral and Maxillofacial Surgery, Osaka Dental University Hospital. Primary culture was started with medium for the epithelium, followed by cloning to be used as ameloblastoma cells. Subsequently, retinoic acid was added at a final concentration of 10-6 to 10-7 M and incubated for 6 hours to prepare an experimental group. A control group was also prepared without its addition. After culturing, the cells were collected to extract RNA from each group for microarray analysis. In the experimental group, the expression of the EDF1 gene was most strongly increased, whereas that of the TM4SF10 gene was mostly markedly suppressed. Furthermore, the expression levels of the MMP3 and FGF2 genes were suppressed by 0.37- and 0.48-fold, respectively. The regulation of cell growth and differentiation comprises comprehensive regulatory mechanisms, such as protein synthesis, degradation, complex formation, and phosphorylation, in addition to gene expression. Our study suggested that changes in the expression of FGF2 and MMP3 genes are partly involved in the inhibition of growth and promotion of differentiation by retinoic acid in ameloblastoma-derived cells. |
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| 学位名 | ||||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 34408 | |||||||||||
| 学位授与機関名 | 大阪歯科大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2020-03-06 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第875号 | |||||||||||
