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Palmitate induces apoptosis and inhibits osteogenic differentiation of human periodontal ligament stem cells
https://osaka-dent.repo.nii.ac.jp/records/261
https://osaka-dent.repo.nii.ac.jp/records/261e693c226-65ae-4527-843d-245df65cb644
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (1.2 MB)
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論文内容要旨・審査結果要旨 (200.5 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2020-04-28 | |||||||||||
| 本文言語 | ||||||||||||
| 言語 | eng | |||||||||||
| タイトル | ||||||||||||
| タイトル | Palmitate induces apoptosis and inhibits osteogenic differentiation of human periodontal ligament stem cells | |||||||||||
| 著者 |
竹内, 友規
× 竹内, 友規
× Takeuchi, Tomoki
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| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Periodontal ligament | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Stem cells | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Palmitate | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Apoptosis | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | Osteogenesis | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Objective: The aim of the present study was to investigate the effect of palmitate on human periodontal ligament stem cells (PDLSCs). Design: PDLSCs were isolated from the third molars of healthy adult donors, and cultured in normal or osteogenic medium supplemented with palmitate (0, 100, or 250 µM) for 21 days. Cell proliferation was evaluated by measuring the amount of formazan at 6, 24, 48, and 72 h. Apoptosis was detected by ELISA and terminal deoxynucleotidyl transferase dUTP nick end labeling assay at days 3 and 7. Osteogenic differentiation was evaluated by measuring the alkaline phosphatase (ALP) activity, production of procollagen type I C-peptide and osteocalcin, mineralization, and mRNA expression of Runx2 at days 3, 7, 14, and 21. In addition, mRNA expression of IL-6 and IL-8 was measured at day 3. Results: Palmitate inhibited the proliferation, ALP activity, production of procollagen type I C-peptide and osteocalcin, mineralization, and mRNA expression of Runx2 in the cultured PDLSCs. Palmitate also induced apoptosis and mRNA expression of IL-6 and IL-8 in the PDLSCs. Conclusions: The results of the present study demonstrate that palmitate induces apoptosis and inhibits osteogenic differentiation of PDLSCs. These findings may help clarify the relationship between palmitate and periodontal tissue regeneration. |
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| 学位名 | ||||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 34408 | |||||||||||
| 学位授与機関名 | 大阪歯科大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2020-03-06 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第878号 | |||||||||||
