| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-04-28 |
| 本文言語 |
|
|
言語 |
eng |
| タイトル |
|
|
タイトル |
Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation |
| 著者 |
黄, 安祺
Huang, Anqi
|
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
catechin｜bone formation｜anti-inflammatory reaction｜matrix metalloproteinase｜biomaterials |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Epigallocatechin gallate(EGCG), which is isolated from green tea, has been shown to have various pharmacological effects. In recent years, we have developed vacuum heated sponge material integrated with epigallocatechin gallate (vhEGCG-GS). Recently, vhEGCG-GS has been proven to have excellent bone regenerative ability. We examined the effects of EGCG on matrix metalloproteinase (MMP)-2 and MMP-9（gelatinases that disintegrate gelatin）expression in gelatin sponges and its association with bone formation. Four kinds of gelatin sponges with or without EGCG were prepared and implanted into bone defects for up to 4 weeks. Histological and immunohistological stainings were then performed. Micro-computed tomography was used to estimate the bone-forming capacity of each group. The results showed that EGCG integration attenuated MMP-2 and -9 expression, increased residual gelatin, and augmented bone formation in critical-sized bone defects of rat calvaria compared with vacuum-heated gelatin sponges without EGCG. In addition, vhEGCG showed superior bone formation compared with other sponges. The results indicated that integration of EGCG in gelatin-based materials modulated the production and activity of MMP-2 and -9 in vivo, thereby enhancing bone-forming capacity. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与機関 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2020-03-06 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第884号 |
学位論文 (5.7 MB)
