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Glucose Affects the Quality and Properties of Hard Tissue in Diabetes Mellitus Model
https://osaka-dent.repo.nii.ac.jp/records/322
https://osaka-dent.repo.nii.ac.jp/records/322bf278b34-5c60-4180-96ed-16c2727583d1
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (4.4 MB)
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論文内容要旨・審査結果要旨 (491.7 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2022-05-13 | |||||||||||
| 本文言語 | ||||||||||||
| 言語 | eng | |||||||||||
| タイトル | ||||||||||||
| タイトル | Glucose Affects the Quality and Properties of Hard Tissue in Diabetes Mellitus Model | |||||||||||
| 著者 |
武, 慶超
× 武, 慶超
× Wu, Qingchao
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| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Bone marrow cells | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Hyperglycemia | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | Stemness | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Hard tissue formation | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Patients with diabetes mellitus (DM) are at an increased risk of tooth loss compared to healthy individuals. Although studies human subjects suggest that diabetes control is affected by periodontitis, there is scarce mechanistic evidence supporting its biological plausibility. Therefore, using type 2 DM rat bone marrow mesenchymal stromal cells (BMMSCs) were incubated with four concentrations of glucose (5.5, 8.0, 12.0 or 24.0 mM), the effects of different glucose concentrations on BMMSCs stemness and osteogenesis were evaluated. High glucose concentrations decreased the fluorescence intensity of β-actin, STRO-1, CD73 and CD90. Moreover, cell proliferation decreased at high glucose concentrations. Alkaline phosphatase activity was decreased at 12.0 mM and 24.0 mM. In contrast, osteocalcin production and calcium deposition were considerably increased at 24.0 mM. Differences in the calcium/phosphate ratio associated with various glucose concentrations were similar to calcium deposition. The mRNA expression of Runx2 and inflammatory cytokines increased with increasing glucose concentration. The RANKL/OPG ratio decreased at high glucose concentrations. A high glucose concentration increased hard tissue formation, but the quality and stemness of the mineralized tissue decreased. Thus, hard tissue had a high risk of bone resorption in the case of uncontrolled diabetes even if periodontal treatment stabilized state of periodontitis for a moment. | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 34408 | |||||||||||
| 学位授与機関名 | 大阪歯科大学 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2022-03-04 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第924号 | |||||||||||
