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膜内ロンボイドプロテアーゼ活性の解析
https://osaka-dent.repo.nii.ac.jp/records/364
https://osaka-dent.repo.nii.ac.jp/records/364557ad6d6-2931-40db-b19e-c2401ee1008b
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論文内容要旨・審査結果要旨 (188.5 kB)
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学位論文 (1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2023-04-04 | |||||
| 本文言語 | ||||||
| 言語 | eng | |||||
| タイトル | ||||||
| タイトル | Intermembrane rhomboid protease activity of Rothia mucilaginosa | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | 膜内ロンボイドプロテアーゼ活性の解析 | |||||
| 言語 | ja | |||||
| 著者 |
麻生, 由樹
× 麻生, 由樹 |
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| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Rothia mucilaginosa | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Rhomboid protease | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Endodontic pathogen | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Virulence factor | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Rothia mucilaginosa is a part of the normal oral microflora, which has been recognized as an endodontic pathogen and is also an emerging opportunistic pathogen in immune compromised hosts. In this study, we identified the rhomboid protease in R. mucilaginosa as a candidate virulence factor. A database search identified two rhomboid genes on the genome with open reading frames of 828 bp and 822 bp, respectively. The two genes are separated by 57 bp, suggesting that they constitute a bicistronic operon. Motifs conserved among rhomboid family proteins (HxxxN, GxSG, and GxxxG) were found in their deduced amino acid sequences. The two recombinant rhomboids and the rhomboid/liposome complexes (proteoliposomes) were prepared using a wheatgerm cell-free translation system, and their serine protease activities were assessed. The two rhomboids showed serine protease activity in the form of rhomboid/liposome complex, while the recombinant rhomboids alone showed little or no activity. On the supposition of host invasion, R. mucilaginosa was cultivated in medium with type I collagen, and the rhomboid mRNA levels were examined. Real-time reverse transcription polymerase chain reaction analysis revealed that the levels of the two rhomboid mRNAs increased slightly in culture with type I collagen, although the changes were not statistically significant. These results suggested that R. mucilaginosa possesses two active rhomboids with protease activity on the membrane, and the two rhomboids may be virulence factors involved in the response to host environmental conditions. | |||||
| 言語 | en | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（歯学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 34408 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 大阪歯科大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | Osaka Dental University | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2023-03-03 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第956号 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2023 The Author(s) | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/ | |||||
| 権利情報 | Creative Commons Attribution 4.0 International | |||||
| 関連情報 | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.30574/wjarr.2023.17.1.1442 | |||||
| 関連情報 | ||||||
| 関連名称 | World Journal of Advanced Research and Reviews. 2023, 17(1), p. 974–983. | |||||
