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経鼻ダブルDNAアジュバントにより誘導されたヒト唾液タンパク特異的SIgA抗体はPorphyromonas gingivalisの付着抑制に必要である
https://osaka-dent.repo.nii.ac.jp/records/367
https://osaka-dent.repo.nii.ac.jp/records/36728d400bd-2eea-46ab-ae6b-50ea11858d53
| 名前 / ファイル | ライセンス | アクション |
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論文内容要旨・審査結果要旨 (209.7 kB)
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学位論文 (1.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2023-04-04 | |||||
| 本文言語 | ||||||
| 言語 | eng | |||||
| タイトル | ||||||
| タイトル | Human salivary protein-derived peptides specific-salivary SIgA antibodies enhanced by nasal double DNA adjuvant in mice play an essential role in preventing Porphyromonas gingivalis colonization: an in-vitro study | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | 経鼻ダブルDNAアジュバントにより誘導されたヒト唾液タンパク特異的SIgA抗体はPorphyromonas gingivalisの付着抑制に必要である | |||||
| 言語 | ja | |||||
| 著者 |
小柳, 圭代
× 小柳, 圭代 |
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| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Statherin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Acidic proline-rich protein 1 (PRP1) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Porphyromonas gingivalis (Pg) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Colonization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Salivary secretory IgA antibody (Salivary SIgA Ab) | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: We previously showed that fimbriae-bore from Poryphyromonas gingivalis (Pg), one of the putative periodontopathogenic bacteria specifically bound to a peptide domain (stat23, prp21) shared on statherin or acidic proline-rich protein 1 (PRP1) molecule of human salivary proteins (HSPs). Here, we investigated whether the nasal administration of DNA plasmid expressing Flt3 ligand (pFL) and CpG oligodeoxynucleotide 1826 as double DNA adjuvant (dDA) with stat23 and prpr21 induces antigen (Ag)-specific salivary secretory IgA (SIgA) antibodies (Abs) in mice. Further, we examined that stat23- and prpr21-specific salivary SIgA Abs induced by dDA have an impact on Pg-binding to human whole saliva-coated hydroxyapatite beads (wsHAPs). Material and methods: C57BL/6N mice were nasally immunized with dDA plus sta23 or/and prp21 peptide as Ag four times at weekly intervals. Saliva was collected one week after the final immunization and was subjected to Ag-specific ELISA. To examine the functional applicability of Ag-specific SIgA Abs, SIgA-enriched saliva samples were subjected to Pg binding inhibition assay to wsHAPs. Results: Significantly elevated levels of salivary SIgA Ab to stat23 or prp21 were seen in mice given nasal stat23 or prp21 with dDA compared to those in mice given Ag alone. Of interest, mice nasally given the mixture of stat23 and prp21 as double Ags plus dDA, resulted in both stat23- and prp21-specific salivary SIgA Ab responses, which are mediated through significantly increased numbers of CD11c+ dendritic cell populations and markedly elevated Th1 and Th2 cytokines production by CD4+ T cells in the mucosal inductive and effector tissues. The SIgA Ab-enriched saliva showed significantly reduced numbers of live Pg cells binding to wsHAPs as compared with those in mice given double Ags without dDA or naïve mice. Additionally, saliva from IgA-deficient mice given nasal double Ags plus dDA indicated no decrease of live Pg binding to wsHAPs. Conclusion: These findings show that HSP-derived peptides-specific salivary SIgA Abs induced by nasal administration of stat23 and prp21 peptides plus dDA, play an essential role in preventing Pg attachment and colonization on the surface of teeth, suggesting a potency that the SIgA may interrupt and mask fimbriae-binding domains in HSPs on the teeth. |
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| 言語 | en | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（歯学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 34408 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 大阪歯科大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | Osaka Dental University | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2023-03-03 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第959号 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2023 The Author(s) | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/ | |||||
| 権利情報 | Creative Commons Attribution 4.0 International | |||||
| 関連情報 | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1186/s12903-023-02821-6 | |||||
| 関連情報 | ||||||
| 関連名称 | BMC Oral Health. 2023, 23(1), 123. | |||||
