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ソーティングネキシン25はマクロファージJ774.1細胞におけるC5a誘導性神経成長因子の発現を調節する
https://osaka-dent.repo.nii.ac.jp/records/368
https://osaka-dent.repo.nii.ac.jp/records/3689d4e5b9c-add7-468d-95da-329c9bdf800b
| 名前 / ファイル | ライセンス | アクション |
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論文内容要旨・審査結果要旨 (191.2 kB)
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学位論文 (719 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2023-04-04 | |||||
| 本文言語 | ||||||
| 言語 | eng | |||||
| タイトル | ||||||
| タイトル | Sorting nexin 25 regulates C5a-induced nerve growth factor expression in macrophage-like J774.1 cells | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | ソーティングネキシン25はマクロファージJ774.1細胞におけるC5a誘導性神経成長因子の発現を調節する | |||||
| 言語 | ja | |||||
| 著者 |
三谷, 早希
× 三谷, 早希 |
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| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Macrophage | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | SNX25 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | NGF | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although the complement cascade is the basis of innate immunity, little is known about its mechanism. NGF acts as an important mediator of the cascade, acting downstream of C5a receptor 1 (C5aR1). Although NGF is known to be involved in inflammatory pain, the mechanism by which it causes pain remains unclear. It is known that an injection of complement 5a (C5a) into the hind paw skin of mice elicits painful behavior and enhances NGF expression in dermal macrophages. Sorting nexin 25 (SNX25) is part of a family of proteins that plays an important role in membrane trafficking, intracellular signaling, membrane remodeling, and organelle motility, some of which affect the immune system. Using the macrophage cell-line J774.1, we examined whether SNX25 is involved in C5a-induced NGF expression at the cellular level. Treatment of J774.1 with C5a enhanced the expression of NGF and SNX25. Western blotting revealed that C5a-induced NGF expression was reduced by SNX25 siRNA. SNX25 +/- mice had lower NGF levels and showed a pain-insensitive phenotype in response to C5a. We propose that the expression level of C5a-induced NGF is controlled by macrophages through SNX25 signalling. | |||||
| 言語 | en | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（歯学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 34408 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 大阪歯科大学 | |||||
| 言語 | en | |||||
| 学位授与機関名 | Osaka Dental University | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2023-03-03 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第960号 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2023 The Author(s) | |||||
| 関連情報 | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.18905/jodu.57.1_55 | |||||
| 関連情報 | ||||||
| 関連名称 | Journal of Osaka Dental University. 2023, 57(1), p. 55-62. | |||||
