| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2016-08-01 |
| 本文言語 |
|
|
言語 |
eng |
| タイトル |
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|
タイトル |
Hybrid scaffold composed of amino-acid coated sponge and hydroxyapatite for hard tissue formation by bone marrow cells |
| 著者 |
薮内, 崇督
Yabuuchi, Takayoshi
|
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
A formalin-treated polyvinyl-alcohol (PVF) sponge is convenient as a scaffold because its configuration is easily modified. However, coating the sponge with an adhesive chemical agent is necessary to attach bone marrow cells (BMCs) to the sponge structure. Moreover, it was considered that a hybrid scaffold composed of a sponge and enveloped cylindrical porous hydroxyapatite (HA) would be convenient. In this study, the effect of leucine (Leu) coating on a PVF sponge was examined for osteogenesis on an HA/PVF hybrid scaffold by rat BMCs (rBMCs). In all in vivo assessment, the sponge immersed in Leu solution (10 mg/ml) was inserted d into the hollow center of cylindrical HA. The sponge received I.5 x 106 rBMCs obtained from male Fischer' 344 rats. The hybrid scaffolds were then implanted subcutaneously of syngeneic rats for 6 weeks. In vitro assessment of Leu to hard tissue formation with coating on the well or addition in rBMC culture medium was also performed in a 6-well plate for 2 weeks. In vivo examinations showed the excellent effect of Leu coating on PVF sponge. Leu-coated PVF sponge in the scaffolds showed marked new bone formation in the pores by histological examination. Leu-coated PVF sponge showed a high qnantity of osteocalcine (OC). HA might prevent the release of rBMCs from PVF as a barrier. In in vitro examinations, the quantity of OC in rBMC culture with and without the addition of Leu in culture medium showed no significant difference. However, addition of Len showed significant ALP activity level in culture medium. Leu coating in culture plate wells showed no influence on the quantity of OC. It was concluded from the results that Leu might prevent the Emigration of rBMCs to the outside of the scaffold and promote the differentiation of cells to osteoblasts in the scaffold. |
| 学位名 |
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|
学位名 |
博士（歯学） |
| 学位授与機関 |
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|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2014-09-19 |
| 学位授与番号 |
|
|
学位授与番号 |
乙第1588号 |
学位論文 (1.1 MB)
