| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2016-08-01 |
| 本文言語 |
|
|
言語 |
eng |
| タイトル |
|
|
タイトル |
The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells |
| 著者 |
⽚⼭, 暢仁
Katayama, Nobuhito
|
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Alkaline Phosphatase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cell Differentiation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cell Proliferation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cells, Cultured |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Collagen Type I |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Dental Enamel |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Flavonoids |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Humans |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mesenchymal Stromal Cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Oligopeptides |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Osteocalcin |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与機関 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2015-03-06 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第744号 |
学位論文 (2.7 MB)
