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High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway
https://osaka-dent.repo.nii.ac.jp/records/178
https://osaka-dent.repo.nii.ac.jp/records/17851432731-51c1-40c5-a2a4-b16828fee723
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (972.6 kB)
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論文内容要旨・審査結果要旨 (199.0 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2018-05-17 | |||||||||
| 本文言語 | ||||||||||
| 言語 | eng | |||||||||
| タイトル | ||||||||||
| タイトル | High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway | |||||||||
| 著者 |
山内, 伸浩
× 山内, 伸浩
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| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | periodontal ligament｜stem cells｜phototherapy｜low-level light therapy｜regeneration｜MAP kinase signaling system | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Background: Light-emitting diode (LED) is attracting attention as a new light source for phototherapy. However, its effects on periodontal tissue regeneration remain unknown. The aim of this study was to examine the effects of high-power, red LED irradiation on human periodontal ligament stem cells (PDLSCs), which play an important role in periodontal tissue regeneration. Methods: PDLSCs were derived from adult human third molars. The light source was red LED (peak wavelength: 650 nm). Energy densities ranging from 0 to 10 J/cm2 were tested to determine the optimal dose. PDLSC proliferation was measured using two parameters: live cell protease and ATP levels. After the cells were induced to differentiate, Next, the effect of LED irradiation on osteogenic differentiation and mineralization was examined, with a particular focus on the extracellular signal-regulated kinase (ERK)1/2 signaling pathway using an ERK inhibitor (PD98059). Results: LED irradiation at 8 J/cm2 led to a significant increase in PDLSC proliferation and enhanced Runx2 and Osterix mRNA expression, ALP activity, procollagen type 1 C-peptide and osteocalcin production, calcium deposition and Alizarin Red S staining. In addition, LED induced the activation of ERK1/2, and the effects of LED on PDLSC proliferation, differentiation, and mineralization could be suppressed by treatment with PD98059. Conclusion: The results of this study show that 650-nm high-power, red, LED irradiation increases PDLSCs proliferation, and osteogenic differentiation and mineralization, mediated by ERK1/2 activation. These findings suggest that LED may be a useful tool for periodontal tissue regeneration. |
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| 学位名 | ||||||||||
| 学位名 | 博士（歯学） | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 34408 | |||||||||
| 学位授与機関名 | 大阪歯科大学 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2018-03-09 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第816号 | |||||||||
