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Rothia dentocariosaに対するトランスポゾンの挿入
https://osaka-dent.repo.nii.ac.jp/records/2000166
https://osaka-dent.repo.nii.ac.jp/records/2000166896eea97-5e43-45d0-af2a-f1809c2b5877
| 名前 / ファイル | ライセンス | アクション |
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論文内容要旨・審査結果要旨 (225 KB)
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学位論文 (918 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2024-12-23 | |||||||||||
| 本文言語 | ||||||||||||
| 言語 | eng | |||||||||||
| タイトル | ||||||||||||
| タイトル | Transposon insertion in Rothia dentocariosa | |||||||||||
| 言語 | en | |||||||||||
| タイトル | ||||||||||||
| タイトル | Rothia dentocariosaに対するトランスポゾンの挿入 | |||||||||||
| 言語 | ja | |||||||||||
| 著者 |
劉, 柏昂
× 劉, 柏昂
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| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Rothia dentocariosa | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Genetic modification | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Transposon insertion mutagenesis | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Objectives Rothia spp. are emerging as significant bacteria associated with oral health, with Rothia dentocariosa being one of the most prevalent species. However, there is a lack of studies examining these properties at the genetic level. This study aimed to establish a genetic modification platform for R. dentocariosa. Methods Rothia spp. were isolated from saliva samples collected from healthy volunteers. Subsequently, R. dentocariosa strains were identified through colony morphology, species-specific polymerase chain reaction (PCR), and 16S ribosomal RNA gene sequencing. The identified strains were then transformed with plasmid pJRD215, and the most efficient strain was selected. Transposon insertion mutagenesis was performed to investigate the possibility of genetic modifications. Results A strain demonstrating high transforming ability, designated as R. dentocariosa LX16, was identified. This strain underwent transposon insertion mutagenesis and was screened for 5-fluoroorotic acid-resistant transposants. The insertion sites were confirmed using arbitrary primed PCR, gene-specific PCR, and Sanger sequencing. Conclusion This study marks the first successful genetic modification of R. dentocariosa. Investigating R. dentocariosa at the genetic level can provide insights into its role within the oral microbiome. |
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| 言語 | en | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 34408 | |||||||||||
| 学位授与機関名 | 大阪歯科大学(ja) Osaka Dental University(en) |
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| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2024-09-25 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第1011号 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2024 Japanese Association for Oral Biology(en) | |||||||||||
| 関連情報 | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| DOI | https://doi.org/10.1016/j.job.2024.04.006 | |||||||||||
| 掲載情報 | Journal of Oral Biosciences. 2024, 66(2), p. 358-364.(en) | |||||||||||
