| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2020-04-28 |
| 本文言語 |
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|
言語 |
eng |
| タイトル |
|
|
タイトル |
Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
| 著者 |
今井, 一貴
Imai, Kazutaka
|
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Shikonin ｜Wound healing ｜Gingival Fibroblasts |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Shikonin is an active ingredient in Lithospermum erythrorhizon that promotes wound healing. We examined wound healing by shikonin using human gingival fibroblasts (hGF) collected from patients. We determined the optimal concentration from the result of cell proliferation and migration. And then we investigated the expression of VEGF, FN and Type I collagen. Moreover, we examined the phosphorylation of ERK 1/2 involved in wound healing. Finally, we performed all experiments using ERK 1/2 specific inhibitor (PD98059).Shikonin 1μM significantly promote cell proliferation and migration, shikonin promoted the expression of VEGF, FN and Type I collagen. Also, shikonin enhanced the activation of ERK 1/2 phosphorylation PD98059 suppressed the these effects of shikonin. In conclusion, wefound that shikonin might be useful in gingival wound healing. |
| 学位名 |
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|
学位名 |
博士（歯学） |
| 学位授与機関 |
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|
|
学位授与機関識別子Scheme |
kakenhi |
|
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学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
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学位授与年月日 |
2020-03-06 |
| 学位授与番号 |
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学位授与番号 |
甲第860号 |
学位論文 (5.8 MB)
