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Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model
https://osaka-dent.repo.nii.ac.jp/records/320
https://osaka-dent.repo.nii.ac.jp/records/3209b047b20-85ea-4fb9-b8ee-edc92f6d2b43
| 名前 / ファイル | ライセンス | アクション |
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学位論文 (1.3 MB)
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論文内容要旨・審査結果要旨 (227.9 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2022-05-13 | |||||
| 本文言語 | ||||||
| 言語 | eng | |||||
| タイトル | ||||||
| タイトル | Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model | |||||
| 著者 |
呂, 金釗
× 呂, 金釗× Lyu, JinZhao |
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| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | stem cells｜Cellnest™｜CellSaic｜ rDPSCs｜rBMSCs｜regeneration｜osteogenic differentiation｜bone repair｜rat congenital cleft-jaw model | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Scaffolds stimulate cell proliferation and differentiation and play major roles in providing growth and nutrition factors in the repair of bone defects. We used the recombinant peptide Cellnest™to prepare the three-dimensional stem cell complex, CellSaic, and evaluated whether CellSaic con taining rat dental pulp stem cells (rDPSCs) was better than that containing rat bone marrow stem cells (rBMSCs). rDPSC-CellSaic or rBMSC-CellSaic, cultured with or without osteogenic induction medium, formed the experimental and control groups, respectively. Osteoblast differentiation was evaluated in vitro and transplanted into a rat model with a congenital jaw fracture. Specimens were collected and evaluated by microradiology and histological analysis. In the experimental group, the amount of calcium deposits, expression levels of bone-related genes (RUNX2, ALP, BSP, and COL1),and volume of mineralized tissue, were significantly higher than those in the control group (p < 0.05).Both differentiated and undifferentiated rDPSC-CellSaic and only the differentiated rBMSC-CellSaic could induce the formation of new bone tissue. Overall, rBMSC-CellSaic and rDPSC-CellSaic made with Cellnest™ as a scaffold, provide excellent support for promoting bone regeneration in rat mandibular congenital defects. Additionally, rDPSC-CellSaic seems a better source for craniofacial bone defect repair than rBMSC-CellSaic, suggesting the possibility of using DPSCs in bone tissue regenerative therapy | |||||
| 学位名 | ||||||
| 学位名 | 博士（歯学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 34408 | |||||
| 学位授与機関名 | 大阪歯科大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2022-03-04 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第922号 | |||||
