Item type |
学位論文 / Thesis or Dissertation(1) |
公開日 |
2022-05-13 |
本文言語 |
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言語 |
eng |
タイトル |
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タイトル |
Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model |
著者 |
呂, 金釗
Lyu, JinZhao
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キーワード |
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主題Scheme |
Other |
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主題 |
stem cells|Cellnest™|CellSaic| rDPSCs|rBMSCs|regeneration|osteogenic differentiation|bone repair|rat congenital cleft-jaw model |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
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資源タイプ |
doctoral thesis |
アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Scaffolds stimulate cell proliferation and differentiation and play major roles in providing growth and nutrition factors in the repair of bone defects. We used the recombinant peptide Cellnest™to prepare the three-dimensional stem cell complex, CellSaic, and evaluated whether CellSaic con taining rat dental pulp stem cells (rDPSCs) was better than that containing rat bone marrow stem cells (rBMSCs). rDPSC-CellSaic or rBMSC-CellSaic, cultured with or without osteogenic induction medium, formed the experimental and control groups, respectively. Osteoblast differentiation was evaluated in vitro and transplanted into a rat model with a congenital jaw fracture. Specimens were collected and evaluated by microradiology and histological analysis. In the experimental group, the amount of calcium deposits, expression levels of bone-related genes (RUNX2, ALP, BSP, and COL1),and volume of mineralized tissue, were significantly higher than those in the control group (p < 0.05).Both differentiated and undifferentiated rDPSC-CellSaic and only the differentiated rBMSC-CellSaic could induce the formation of new bone tissue. Overall, rBMSC-CellSaic and rDPSC-CellSaic made with Cellnest™ as a scaffold, provide excellent support for promoting bone regeneration in rat mandibular congenital defects. Additionally, rDPSC-CellSaic seems a better source for craniofacial bone defect repair than rBMSC-CellSaic, suggesting the possibility of using DPSCs in bone tissue regenerative therapy |
学位名 |
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学位名 |
博士(歯学) |
学位授与機関 |
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学位授与機関識別子Scheme |
kakenhi |
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学位授与機関識別子 |
34408 |
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学位授与機関名 |
大阪歯科大学 |
学位授与年月日 |
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学位授与年月日 |
2022-03-04 |
学位授与番号 |
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学位授与番号 |
甲第922号 |