| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2022-05-13 |
| 本文言語 |
|
|
言語 |
eng |
| タイトル |
|
|
タイトル |
Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model |
| 著者 |
妻野, 誠仁
Nsumano, Nobuhito
|
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
bone regeneration |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
alfa-tricalcium phosphate |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
animal study |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
dedifferentiated fat cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
rat calvar-ial defect |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Mechanical and resorbable scaffolds are in high demand for stem cell-based regenerative medi-cine, to treat refractory bone defects in craniofacial abnormalities and injuries. Multipotent pro-genitor cells, such as dedifferentiated fat (DFAT) cells, are prospective sources for regenerative therapies. Herein, we aimed to demonstrate that a composite gelatin sponge (α-TCP/GS) of al-fa-tricalcium phosphate (α-TCP) mixed with gelatin scaffolds (GS), with/without DFATs, induced bone regeneration in a rat calvarial defect model in vivo. α-TCP/GS was prepared by mixing α-TCP and 2% GS using vacuum-heated methods. α-TCP/GS samples with/without DFATs were transplanted into the model. After 4 weeks of implantation, the samples were subjected to mi-cro-computed tomography (μ-CT) and histological analysis. α-TCP/GS possessed adequate me-chanical strength; α-TCP did not convert to hydroxyapatite upon contact with water, as deter-mined by X-ray diffraction. Moreover, stable α-TCP/GS was formed by electrostatic interactions, and verified based on the infrared peak shifts. μ-CT analyses showed that bone formation was higher in the α-TCP/GS+ DFAT group than in the α-TCP/GS group. Therefore, the implantation of α-TCP/GS comprising DFAT cells enhanced bone regeneration and vascularization, demon-strating the potential for healing critical-sized bone defects. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与機関 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
34408 |
|
|
学位授与機関名 |
大阪歯科大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2022-03-04 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第938号 |
学位論文 (1.9 MB)
